re-staining after IHC of mouse brain slices - (Jan/29/2015 )

Hi together,

I work with 30 µm thick mouse brain slices HC-area.  We performed IHC doublestaining BRDU/DCX to count double positiv cells in the DG-region. In some slices the DCX-staining didn't work, better, the staining is really weak. Is it possible to restain these slices maybe with a higher concentrated 1. AB solution or should be an AB-destaining performed before?

Thanks for your help!

cu Funny-Fish

Hi Funny Fish, I don't want to discourage you, but I have attempted such thing and has never worked for me. But who knows it might work for you.

If you have enough unstained sections with you, it is always best to do a clean experiment.