FACS with culture media - (Jan/15/2015 )

can i run a FACS analasys with cells in culture media?

Yes, you can.

If your medium has phenol red indicator (as most do), that might decrease the sensitivity a bit because the increase in background fluorescence will change the system baseline for signal levels. But not a big deal generally.

To be honest, I would not recommend it because of the increase in background fluorescence to be expected. How strong is your positive signal ?

and just curious, why do you want to do that ?