Blocking buffer for biotin-labeled EMSA - (Dec/22/2014 )

Hi.

Can someone please advice a good blocking buffer for the detection step of an EMSA using biotin-labeled probes?

Thanks in advance!

Basically, anything with lots of diverse protein except milk. Milk has biotin. I believe there are commercial products made from fish protein, such as Pierce Sea Block.

Thanks. I tried this buffer...

• 7.3g NaCl,
• 2.4g Na₂HPO₄
• 1g NaH₂PO₄
• 50g SDS
• dH₂O to 1 L

but the membrane was very dirty when I developed it, so I guess this one is not good.