site directed mutagenesis - (Dec/19/2014 )

Hi all, can anyone explain how you would do site directed mutagenesis to a protein? Are there any kits for that ?

tnx

You do it on the DNA (e.g. on a plasmid). Qickchange is the most commonly used version, but there are a number of others around too.

I agree, Quickchange is probably the most common. In-Fusion from Clontech is an alternative. The idea behind site-specific mutagenesis is normally to align primers to a plasmid with your gene of interest that have a mismatch at the codon you want to mutate and do a PCR. The original plasmid (which you purified from bacteria) can then be digested with a methylation sensitive restriction enzyme, while the new version that you synthesized by PCR stays intact.