Ligation and blue white screening - (Nov/28/2014 )

Greetings

Im working with two pTZ57/R plasmids (A and B). Each plasmid had a fragment inserted into its MCS therefore the beta-galactosidase gene has been interrupted. next I then cut a portion of each pTZ plasmid, this portions contain the insert and part of the pTZ sequence, i then ligate the two portions together. Now, after transformation, do i plate onto IPTG XGAL plates or just normal plates, becasue i mean the beta galactosidase gene has already been interrupted so there wont be any blue colonies formed right? so i can just go ahead and plate on ordinary amp plates? 

thanks

Without seeing the sequence it is hard to tell, but it sounds like you can just plate on regular plates.