elisa detection limit and sensitivity - (Nov/22/2014 )

Are lower detection limit and sensitivty same thing for elisa? how absorbance detection limit can be calculated from a 4 Parametric logistic curve and then convert it to the the analyte concentration detection limit?

Please someone give me the answer. I need to know this.

Yes and use the equation...

Bob1 thanks for ur suggestions. Can u please tell me a little details how to get LOD conc. using equation.

Bob1 thanks for ur suggestions. Can u please tell me a little details how to get LOD conc. using equation.

The equation tells you the X and Y values for the line drawn by your standard curve. Solve the equation for the respective values of X based on value Y.