Visualizing GFP in transgenic rice seeds? - (Oct/20/2014 )

I want to express GFP in rice as a method for differentiating transgenic from non transgenic rice seeds. I want to know if I can visualize them using a hand held UV lamp as the GFP I will be using has a excitation maxima at 395nm. If yes, how is to be done? Do I just flash the UV lamp on the seeds in the dark room and I can see the GFP expressing seeds glowing green?I am using mgfp5, optimized for plant expression which has excitation maxima at 395nm and 473-477nm(blue).What should I do? Cana Hand held LED torch with Blue LED of 473nm help in visualizing the transgenic seeds?

This depends on where the GFP is expressed - I doubt it would be in the protective shell (Palea? Lemma?), but is more likely in the germ-plasm (you should probably know these details) - you may need to germinate them to differentiate between transgenic and non-transgenic.

With those wavelengths you would probably want to avoid UV (I would avoid it anyway, it could damage the DNA) and go for a short wave-length blue light, such as a blue transilluminator used for looking at DNA gels.

You could possibly also put the seeds in a 96 well plate and pass them through a fluorescence microplate reader.