Making a fusion protein - (Aug/20/2014 )

Hi guys

At the moment I have a bit of a concern

I was wondering if any one had a spare mement and could explain to me

why do we need a string of AAAAAA, on our foward and reverse primers

I am cloning a fusion protein and am in the primer design phase

Is there a reason for the A's????|

Woudl appreciate any feedback

Yours Sincerely

Liam

Where on the primers do you need this string?

There are usually only three components you need in a primer that will make a product for digestion and subsequent cloning. You need a specific sequence targetedto the sequence you want to amplify, a restriction site, and some bases 5' of the restriction site to give the RE something to bind to. This last bit is possibly what you are referring to.