animal cell culture - (Jun/27/2014 )

Iam maintaining retinal ganglion cell line-5 for my glaucoma work. I would like to maintain these cell lines at high pressure in the lab. Could anyone help me out in devising this technique?

I have used this model with primary RGCs. Please
contact David Calkins and Rebecca Sappington
at Vanderbilt University. They are experts in in vitro modeling of high pressure glaucoma. You have my permission to drop my name. Please advise of your success.