Methanol concentration for cells? - (Jun/23/2014 )

Hello,

I will solve a drug to use methanol for HT-29 cell line. Final concentration for methanol is 0,3 %. Is it toxic for the cell line? Can I use the drug at 0,3 % final concentration?

-medibio-

The answer is "Maybe" 0.3% will probably be fine, but it depends on the cell line a lot. Note that if the drug is soluble in methanol, it will also be soluble in ethanol, which might be less toxic.

-bob1-

if the drug is soluble in methanol, it will also be soluble in ethanol

Is it accurate? If Ok, I can use EtOH.

-medibio-

Methanol and Ethanol are very similar chemicals - the only difference is a CH3 group, so they behave in the same fashion as other members of the alcohol group.

-bob1-

I recommend a titration of your selected solvent and assess toxicity by yor
favorite assay. Alternatively, many drugs are available as water soluble salts or
in pharmaceutical encapsulations (eg., PEG or

-mlomonaco-