washing pellets; recovery of isolates in glycerol - (Jun/13/2014 )

it started by using 10 ml based on our lab technician initial "say" and i noticed in some papers they used such volumes maybe to prepare enough inoculum, and somehow i did the same. But then with time i realized i don't need 10 ml! 5 ml would have been sufficient.

But whats the goal? Just to inoculate a larger volume the next day?

You might simple use 3 ml, and take 2-3 tubes...

If the liquid is too high, it will not reach the needed OD..

I am not sure what you are doing in the end, so perhaps the 10ml will do the trick too.

yes that's it to have a larger volume...you are right , i could have done the way you are saying.  this is why i was questioning if i change to lower volume will that change my expected cfu/ml at a specific OD.

I like what i am being through in this PhD, few unneeded procedures i am doing, yet i am learning.

Thx always!!

I would just use 3ml than rather than 10.
5ml is still ok.. but 3ml is what most people do.

yes..i will !