Transfection Enhancer Efficiency - (Apr/30/2014 )

Hi All, 

There are a couple of transfection enhancers available for lipid-based transfection, nucleobooster (BCC), Effectene (Qiagen) and Lipofectamine Plus (Life Technologies). Does anyone have experience working with them? And what is the transfection efficiency and toxicity with these enhancers?

Edit - You posted this same question in three different subforums. There is no need for that. New questions are displayed on the homepage and will be viewed/answered regardless of the subforum that are in. -JerryShelly1

Hi,

for siRNA/miRNA as well as for plasmid/shRNA/mRNA transfection I use a new kind of transfection reagent. Its called: Viromer and was developed from Lipocalyx (www.lipocalyx.de).

Viromers are synthetic polymers zero in charge and very gently to cells. Due to an active endosome escape mechanism I get higher transfection efficiency compared to standard transfection reagents.

Please find attached some data and information.