PMSF stability in RIPA buffer - (Apr/07/2014 )
I have used RIPA buffer with PMSF for cell lysis of some salivary cells (cheek cells). But I have some extra RIPA buffer left over with PMSF in it. I have stored it in the deep freezer compartment. Will the PMSF get degraded, since PMSF is unstable in water (aqueous medium)? Also for how long can I store?
Looking forward to a quick reply.
Thank you in advance.
-Biologist91-
pmsf is very short lived in water (on the order of 30 minutes). if you want to use your stored ripa then you'll have to replenish the pmsf.
-mdfenko-
If there is any residual PMSF won't the concentration change?
-Biologist91-
there will probably not be any residual pmsf but, even if there is, it shouldn't make a significant difference.
-mdfenko-
oh alright.
-Biologist91-