mRNA copies of each gene under different promoters - (Mar/20/2014 )
Hi;
how may copies of mRNA do you get from each plasmid in mammalian cells, transient transfection, under different promoters?
I am looking for some comparison, eg. how many copies of mRNA from each gene under CMV versus CAG or SV40 or whatever else.
I found this paper http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.0040309 that seems to suggest that number of copies of mRNA is around <50, but I dont know what to think about it.
-kate-hamster-
This is one of those things that is virtually impossible to measure, as it is very hard to determine how many copies of the plasmid are present in a cell, though in theory this can be measured by qPCR. So - short answer: you aren't likely to find this information.
-bob1-