How to calculate MOI? - (Mar/17/2014 )

Hi every one,

I need to design an experiment and I need to Transduce my cells with vector dilution MOI 50.

Number of my cell is 5x10e5

Virus titter is 10e8

I am not sure how to calculate MOI, could you please help me with this one.

Cheers

MOI is multiplicities of infection - a moi of 1 would mean that every cell receives 1 infectious unit, a moi of 50 is 50 x that number...

5 x 10^5 *50 =??? (this is n in the following equation)

then use v=n/c to get the volume of your virus that you require.