PCR that leads to protein synthesis - (Feb/14/2014 )
pulse of electricity from neuron makes the next neuron fire so I think that doing the same thing to my cell would make it come to life. Now yes ATP is important but so are ions.
I 'spect you need a large pulse -- perhaps from a lightning bolt. Be sure to tie the proto-cell down well to the table, else it might become violent and escape.
caters on Sun Feb 23 02:08:29 2014 said:
pulse of electricity from neuron makes the next neuron fire so I think that doing the same thing to my cell would make it come to life. Now yes ATP is important but so are ions.
Yes - that's an action potential - the next neuron is already alive and functioning before the pulse hits, it isn't the pulse that brings it to life.
Some ATP will be in the cell from an injection just before I send the action potential and then it will go do lots of glycolysis and fatty acid and amino acid metabolism to really raise the ATP levels.
Just ATP though doesn't bring it to life either so it is important that not only does the cell have ATP but also have an action potential sent by the electrode.
And Phage434 just an action potential will do it. In fact lightning would kill your cells unless you have AF or VF(atrial fibrillation and ventricular fibrilation), then it would act like a defibrilator making your heart go back to normal.
As an aside, do you know how much voltage a lightning has ? Up to 200 Million Volts. Do you know how much voltage is applied at defibrillation ? Around 2000-4000 Volt. A lightning strike often induces VF or asystolia, which is one of its deadly mechanisms, and a lot more thermic damage. It does not "make your heart go back to normal" when you have AF/VF.
On another note, when experimenting with electricity, please always consider your safety.
It is neuron strength electricity I am sending to the cell. The maximum that action potential could so is that FES where they use electrodes to make certain muscles contract.
caters on Sun Feb 23 23:37:35 2014 said:
Some ATP will be in the cell from an injection just before I send the action potential and then it will go do lots of glycolysis and fatty acid and amino acid metabolism to really raise the ATP levels.
Just ATP though doesn't bring it to life either so it is important that not only does the cell have ATP but also have an action potential sent by the electrode.
My point is that electricity will not magically make a cell come alive...
You may be interested in this from biotechniques:
Hmm that is interesting that those gaps on the chromosomes aren't gaps but rather rRNA encoding regions.
The nucleolus will probably form when I get the diploid set of chromosomes into the nucleus but I am not positive on that since they have been separated in test tubes for a long time. It might even be forming now as the diploid set is in a test tube of its own.