AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (Jan/27/2014 )
Thanks a lot. If possible, can you share a link of your publication please?
-Nilufer-
This one.
It's only a brief report though, so there wasn't much space to discuss it, but it cites this paper, that originally showed that AZA is not the cause of increased expression of gamma-globin (which is silenced by hypermethylation in adults):
Mabaera R, Greene MR, Richardson CA, Conine SJ, Kozul CD, Lowrey CH. 2008. Neither DNA hypomethylation nor changes in the kinetics of erythroid differentiation explain 5-azacytidine’s ability to induce human fetal hemoglobin. Blood
111:411–420.
-Trof-