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Reading Oligonucleotides Information - (Jan/21/2014 )

Good evening. I need a help to read the information on the primer information sheet. 

What is the different between Tm(1M Na+) and Tm(50mM Na+)? 


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-Lelamekala Vengidasan-

a factor influencing melting temperature is ionic strength of the solution (and the specific ion).

-mdfenko-