cell-cell contact in transwell systems - (Jan/19/2014 )

I would like to conduct transwell experiments with two cell types and wants to investigate the effect of cell-cell interaction between these 2 cell types.

Do anyone have protocol of doing co-culture of these cells in the transwell system (ie. cell type 1 on the upper surface of the insert; while cell type 2 on the lower surface of the insert).

Thank you very much.

Check out the "vascular cell coculture" page on this link:

https://www.cvrc.virginia.edu/Isakson/methods.html

This is a method for coculture of endothelial cells and vascular smooth muscle cells on both sides of a transwell in order to replicate myoendothelial junctions (ie: direct contact between the two cell types) within the pores of the transwell.  I don't know if this protocol could apply to your cell types, but it might give you an idea of where to start.

Good luck!