how to post fix frozen brain sections - (Jan/13/2014 )
I have human brain tissue frozen at -80 C.
I will be sectioning it using OCT and a cryostat so tissue will stay frozen while sectioning.
How do I go about fixing the sections?
Should I use PFA or formalin?
How long should the sections be fixed for?
Any info would be helpful!
-kdf044-
for all intents and purposes. pfa becomes formalin when solubilized (see this wikipedia page).
so, if that is what plan to use then use formalin to fix.
-mdfenko-