Can serum free medium inactivate trypsin? - (Dec/03/2013 )

hi, i am first time in forum,

my concern is,

can serum free medium inactivate trypsin?? i didnot have this idea so mistakenly i used serum free medium to inactivate trypsin which resulted transfected cells(gfp +ve) lost gfp(confirmed by florescence microscope) but still cells are alive. can anyone help pls.

Short answer, no.  Long answer: maybe, but it will depend on the additives in the medium.