rna isolation from formalin fixed peg tissue - (Nov/14/2013 )
a friend of mine posted this question at another forum and hasn't received any responses.
i would appreciate it if any of you can answer his question:
"Is anyone aware of any protocol for isolating RNA from formalin fixed polyethylene glycol embedded tissues?"
thanks
-mdfenko-
No specifics, but I think the PEG will probably preserve the RNA well. I would attempt to just use a protocol for formalin fixed tissue, and do a bunch of washes beforehand to remove the PEG.
-bob1-
thanks, bob1. i'll make sure my friend sees your reply.
-mdfenko-