Problem in Determination of Total Bacterial Cells - (Nov/10/2013 )

Yup. I am planning to use hemacytometer as well. But I just wondering what type of staining dye I should use to stain both live and dead cells. Thanks. 

P/s: They are gram-positive bacteria

You might try trypan blue. Search google scholar, I'm sure you'll find some applications tho' nota sure with a hemocytometer.

You are right. Trypan blue is common use, but I also consider whether it is capable to penetrate trough peptidoglycan cell wall? 

Literature says it serves well for staph viability. try it.