No amplification with TRAPEZE kit! - (Nov/08/2013 )

I have been using a TRAPEZE kit (S7710) and I seem to be having some problems. When I run my samples I get no amplification at all. This includes from the standard curve samples in the kit, the positive control provided and my own positive controls. 

 

I have been using an ABI 7500 machine which is fully functional and I have checked the cycling parameters which are correct. I have been using Titanium Taq which works fine elsewhere and the plates I am using are optically clear. This kit has been used by others before me and worked fine. 

 

I am concerned because the kit is a few years old, so could it be the case that the reagents have gone off? The preparation for the experiment has run smoothly so I can't think of what else could be going wrong and I'm now running out of reagents! 

-Lcc1844-

-bob1-