DNA extraction impurities - (Nov/02/2013 )

I honestly don't know. EDTA is a good anticoagulant. Maybe try contacting Promega and see if they have any advice.

jerryshelly1 on Mon Nov 18 14:18:02 2013 said:

I honestly don't know. EDTA is a good anticoagulant. Maybe try contacting Promega and see if they have any advice.

I have contacted them so many times, they tried to help me with many suggestions but they didn't work out

Thank you for your effort with me, obviously my case is hopless, hail to science X:-(

It may be worth it to contact another company and see if you can try one of there kits for free. Since you regularly isolate blood, it will be beneficial for them. I have had good success with EZ Bioresearch's MW blood isolation kit (competitively priced).

It makes me worried that two people have commented on the inefficiency of this kit.

jerryshelly1 on Mon Nov 18 15:01:21 2013 said:

It may be worth it to contact another company and see if you can try one of there kits for free. Since you regularly isolate blood, it will be beneficial for them. I have had good success with EZ Bioresearch's MW blood isolation kit (competitively priced).

 

It makes me worried that two people have commented on the inefficiency of this kit.

It works perfectly for the the day old samples, but anything older than that gives varying results, which includes some horrible results, and sometimes I cannot go to the lab and extract DNA each time I get a sample, so I need 2-3 days to have full patch since the lab is far.

I cannot change the kit now because I have included it in the thesis proposal and IRB forms

Thank you very much for your effort