Using NCBI to filter results to retrieve one file for each of the several differ - (Oct/26/2013 )
Hi I'm a third year student who is doing Bioinformatics as a module at university. I'm enjoying it and want to continue it later. Anyway my question is:
I was given the following questions which I'm having trouble understanding:
I need to use the NCBI database to retrieve ONE file for each of the several forms of ferritin found in humans. Each file should contain the complete mRNA sequence.
So I went to NCBI and did a search ' Human Ferritin' and put nucleotides tab on the right.
I got 5038 results
The second result said 'Human ferritin Heavy chain mRNA complete cds'
I clicked on the link to display 'related sequences'
Brought the results to 170
I saw things like gorilla in the results so I want to filter even more?
I can click the Homosapiens filter to get it down to 72? But do I want human or homosapiens because are they not the same thing?
The results has a mixture of human and homo etc but how can I just get it to display human?
And how can I filter the results to just display 'the complete mRNA sequence'
I have done some reading and one book says' you can limit the results by excluding 'EST' as these are partial sequences but on my tab I don't have that option I just have limit STS etc
The question says one of the several types of ferritin found in humans. Several means ' More than two but not many' right? but I have so many results which I can't filter down even more.
I't cant be that many because we need to compile a table and compare the mRNA sequence elements after.
I don't want someone to do my work for be, I want to learn how to do it myself as I'm dyslexic and have trouble interpreting questions sometimes
luke
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