MTT assay - (Oct/24/2013 )

HI

I have two question about MTT assay

1. The medium for MTT assay should been added FBS?

    Someone told to me that FBS can help cell proliferation. Will it effect the accuracy?

2. When i read papers about MTT assay,i found many experiments that treat cells in the same dose of drug for different times.

    What are the meaning of different treatment times? (eg. 3, 6, 12, 24 hours)

    Do the different times mean acute or chronic toxicity?

Thank you

1- when you culture cells in 96-well plate you must have FBS, but when you treat them the next day it's up to you to use it or not. Less FBS means less cell growth. 

2- it's called time point study. It's not only for MTT, you can do this for any type of experiment.