DNA extraction , phenol , ice . plz help ! - (Oct/13/2013 )
hi every one , i wanna know why we put our solution on ice after we add phenol to it .
any help is appreciated
XOXO
-M_shabani_medbiotech-
As far as I know there isn't any need to ice during the phenol step (unless perhaps you are working on something with a lot of DNases). I have done many a DNA phenol extraction where the only time I would use ice is during the DNA precipitation.
-bob1-
You do not need to keep on ice you get better separation at RT. Even for precipitation RT works better.
-Shefali Desai-
my supervisor told me so
Thanks guys
-M_shabani_medbiotech-