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hybridization probe - (Sep/16/2013 )

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...

-fd_HeLiX-

http://www.lifetechnologies.com/my/en/home/references/protocols/nucleic-acid-amplification-and-expression-profiling/pcr-protocol/pcr-and-rt-pcr.html

 

or check here for more info

 

http://www.protocol-online.org/prot/Molecular_Biology/PCR/Real-Time_PCR/index.html

 

FAM, JOE and Rhodamine are quite popular

-Curtis-