hybridization probe - (Sep/16/2013 )
I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...
-fd_HeLiX-
or check here for more info
http://www.protocol-online.org/prot/Molecular_Biology/PCR/Real-Time_PCR/index.html
FAM, JOE and Rhodamine are quite popular
-Curtis-