Cytokines feeding cell culture - (Sep/14/2013 )
Hi everybody,
I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.
If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.
I would appreciate your comments! thanks!
I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.
If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.
I would appreciate your comments! thanks!
-yyc-
Usually no of cells seeded per well depend on the surface area of the well; I ue 1e5 per cm2; the vol of media depends on the duration of the culture; finally, amount of cytokine usually depends on the vol of media added.
-Tarique-