Blocking a protein in cell culture through antibody - (Aug/18/2013 )

Hi, yes, of course I know that the antibodies I tried were all against the extracellular domain. I don't know a lot about permeabilization, but a quick google search for "permeabilize living cells" gave some results so maybe it's possible, but I would not recommend it as it could interfere with a multitude of processes and affect your blocking results.

The more obvious way would be to buy an antibody against the extracellular domain of your membrane receptor (or is no such antibody available?).