Difference between PFA, acetone and methanol in cell fixation - (Aug/12/2013 )
Does anyone know what are the differences between these fixatives? I've tried looking it up on Google but couldn't find a comprehensive guide on this. Some people use methanol to fix their cells for certain antibodies but others use PFA.
-science noob-
http://www.abcam.com/index.html?pageconfig=resource&rid=11462
And more info about mechanisms of aldehides fixation reactions:
http://publish.uwo.ca/~jkiernan/formglut.htm
-El Crazy Xabi-