dissociation curves - (Aug/06/2013 )
Please find the attached files for dissocoation curves from qpcr
do they look fine????
please suggest if they are not good.
thanx in advance
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-harry348-
No. That is typical of an instrument issue. You also have some high temp peaks indicative of mis-priming. That's usually due to primer design. Also, your Supermix can cause problems if its not ideal. Check out this tutorial for Supermix help. Www.bio-rad.com/supermixes_tutorial.
-traveler9907-