Referencing pAMP sequence in BLAST - (Aug/02/2013 )

All,

I need your help. This may be an elementary BLAST question that may have been covered, but I searched here and could not find it.

I took the pAMP sequence from the CSH: DNA Learning Center: http://www.dnalc.org/resources/plasmids.html

and plugged it into BLAST http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch

The results do not match the coverage for pAMP, I'm getting results for pUC cloning vectors.

Am I doing something incorrect?

-centralamgod-

I think what you got is right. Probably, according to this site: bhhs.bhusd.org/ourpages/auto/2012/1/.../Amgen%20lab%20handout.doc‎

The plasmids, pAMP and pKAN, were engineered by the DNA Learning Center at Cold Spring Harbor Laboratory. The pAMP plasmid was engineered from the pUC19 plasmid which contains amp^r and a 1904 bp restriction fragment cut from 8 DNA.

Actually you can blast again the vector database http://www.ebi.ac.uk/Tools/sss/ncbiblast/vectors.html

-pcrman-

I thought that because of the prevelant use of these plasmids (pAMP and pKAN), their entire sequence would register in BLAST.

Thank you Mod! This is helpful.

-centralamgod-