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Western Blot-No Sample Band, but Positive Control band visible - (Jul/23/2013 )

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actin will polymerize in relatively low concentrations of salt. we routinely polymerize in 100mM and can get it to polymerize in 50mM.

 

you may be pelleting the actin before you take the supernate for the gel.

 

have you checked the pellet for actin?

-mdfenko-
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