Labeling and tracking RNA - (Jun/04/2013 )

Hi,

I am trying to find a way to fluorescent-label RNA molecules and track them in the cell by microscopy. My idea is to synthesize the RNA (in-vitro transcription) and transfect them into the cell. I know that you can incorporate fluorescent labeled dNTPs into the RNA (like the way one would synthesize RNA-FISH probes), but is the RNA structure/function would be unaltered? Is there any method for adding a fluorescent label to the end of the RNA, without messing with the main portion of it?

Thank you for your time

K

-kaveh-

check this webpage, it may contain the information you want:

life technologies, molecular probes, rna labeling

-mdfenko-

This seems appropriate:
http://www.sciencemag.org/content/333/6042/642.short

-doxorubicin-

These kits are probably more economical: http://www.markergene.com/oliglo2.php

-doxorubicin-