elisa OD calculation - (May/08/2013 )

Hi,

I need to measure TNFalpha secretion in the cell supernatent. The final volume is 0.5ml. The OD of the standards is pg/ml so do I need to halve the OD's of the test samples as the final volume is 0.5 ml.

Thanks.

No, absorbance is a function the pathlength of the light though the sample in the well (see Beer-Lambert law - so if you loaded 100 ul into each well for both standards and samples then there isn't a problem, results should be directly convertable.

Thanks a lot.