Normalization factor - (Apr/06/2013 )
Hello,
could you please suggest me some software/excel sheet/formula to calculate normlaization factor for qPCR data?
-Mad Researcher-
Maybe this helps:
A new mathematical model for relative quantification in real-time RT-PCR.
Pfaffl MW.
Nucleic Acids Res. 2001 May 1;29(9):e45
-cellthetruth-
On this (by the way excellent) real time pcr tutorial website you can find the link to an excel sheet using the Pfaffl Method:
http://pathmicro.med.../pcr-pfaffl.htm
And here is the tutorial itself:
http://pathmicro.med...altime-home.htm
-Tabaluga-
Thanks guys
-Mad Researcher-