Nested PCR - (Mar/22/2013 )

Hello,

I will be attempting a PCR and I was wondering how much of my reaction with my first primer set I should add to the second reaction. I've also read that an exonuclease can be added before the second reaction to eliminate anything that is left of the first primer set. Is this necessary?

Thank you.

have a look here: http://www.protocol-online.org/biology-forums/posts/30427.html

Great, thank you for the link!