How this formula is derived ? - (Mar/18/2013 )

How the formula is derived from which Concentration of a molecule in Serum is determined with the help of Optical Density of serum , standard and control measured by colorimetry ?

hey Rajpat -

this may help you: http://www.ncbi.nlm.nih.gov/pubmed/20495550

also, can you tell us more about what you are trying to measure? can you please give some specifics about your compound of interest?