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Cloning at 6 kb gene - (Mar/07/2013 )

Hello all,
Im trying to Clone a 6KB gene from flower cDNA.Any suggestions before i actually start the work?Im not sure if its gonna be easy amplyfing a 6kn fragment.Is there a good solution to clone this?
Thanks!

-praveen921-

use a high fidelity proofreading polymerase in your pcr

-mdfenko-

Hi i used two polymerases in separate reactions to amplify the 6KB gene
1.Pfu Ultra II DNA Polymerase

2.PrimeSTAR HS DNA Polymerase

I followed the PCR conditions they mentioned in the protocol,but i wasnt able to amplify the gene.What should i do now?
thanks!

-praveen921-

check that your primers are appropriate.

-mdfenko-