Sodium orthovanadate (Na2VO3) preparation - (Feb/15/2013 )

Anyone prepared Na3VO4 to be used as a component of the protein lysis buffer before?

It seems like the pH has to be readjusted to pH8 using a tricky protocol.

And I heard you would have to activate it prior to usage by boiling?

do you mean na3vo4?

see this webpage:

sodium orthovanadate activation

mdfenko on Fri Feb 15 13:02:06 2013 said:

Do you have to reactivate it after thawing out frozen aliquots?

I found that some aliquots appear liquid at -20C and dont freeze. Would you know what is it so?

science noob on Fri Feb 15 13:52:26 2013 said:

check the pH, if it's low then yes, but, since the activation procedure says to store aliquots of active orthovanadate at -20C, you probably won't have to.

not a clue (maybe it's concentrated enough to suppress the freezing point to lower than -20C, maybe your freezer is off, maybe you looked during defrost cycle?)