chemical-induced HCC mouse model - (Jan/29/2013 )

Hi,

I've never worked with mouse models but have to plan for one and would appreciate any advice.

I looking to develop a mouse model that forms HCC after cirrhosis. I've read a few papers and most use CCL4. Has anyone used this treatment and if so:

1. howl ong doe sit to take to develop cirrhosis
2. how long doe sit take to develop HCC following cirrhosis
3. the % of survical of mice. Can teh mice survive til the development of HCC
4. Is this a harsh treatment?

Many thanks

I found some old Italian papers, might be helpful?

http://www.ncbi.nlm.nih.gov/pubmed/7959573
http://www.ncbi.nlm.nih.gov/pubmed/8358289