isolating DNA from cells in suspension - (Jan/13/2013 )
When isolating DNA from cells in suspension, most if not all the protocols I have come across recommend centrifugating the suspension at the very beginning to obtain a cell pellet, followed by resuspension of the pellet in either water or PBS or other buffers.
May I know what is the purpose of obtaining this cell pellet?
Can we just vortex the suspension and use it directly without pelleting the cells?
It would be so that you can concentrate your cells into a much smaller volume to facilitate the DNA extraction.
leelee on Sun Jan 13 13:39:18 2013 said:
It would be so that you can concentrate your cells into a much smaller volume to facilitate the DNA extraction.
Oh..so if the protocol recommends us to resuspend the cell pellet into 200ul of buffer, and I have an initial cell suspension volume of 200ul, does it mean that I don't have to perform the centrifugation and resuspension?
You don't really want to be doing an extraction in your cell culture media, so resuspend in buffer like the protocol states.
Getting rid of unnecessary molecules in media. And handling with less solution (concentrating).
Thanks for the feedback!