Wet transfer of proteins between 100-200kDa - (Jan/11/2013 )

Hi I was wondering what voltage/time others have used with PVDF membranes to transfer proteins of this size efficiently? Thanks.

I think 120V for 1.30 - 2.00 h. Maybe should put PVDF in methanol first before transferring. Always check your protein ladder/marker.

Low and slow is always best for a wet transfer. I use 15-30 V overnight. Higher voltages cause problems with heating. Minimize the amount of methanol you use and you can add some SDS to increase mobility.