Alizarin Red Staining on scaffolds... - (Jan/10/2013 )

Hi all, I'm planning to use alizarin red staining assay to observe and quantify bone formation on my scaffolds.. Will it be the same procedure as being done on a plate/well. For quantification purpose, we use the de-staining solution and take results using a plate reader. Is it necessary to scrape cells? I cannot scrape cells from a scaffold.

Thank you

P.S. : I'm new to tissue eningeering..

And I also want to know what wavelength light should be used for 'quantification' of the de-stained solution (using Cetylpyidinium chloride) in plate-reader. And also how to interpret the results (the values from the reader)...

Thank you