What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (Jan/02/2013 )
Dear,
For expression of GFP, one used
Thank you in advance.
-lioiviei14-
lioiviei14 on Thu Jan 3 03:25:21 2013 said:
Dear,
For expression of GFP, one used
Thank you in advance.
It helps to add the polyA tail to the upstream sequence and acts as a transcriptional terminator sequence. Here is a recent paper describing its effects:
http://www.ncbi.nlm.nih.gov/pubmed/22306880
-JYaron-