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What is the role of SV40 sequence in "5'UTR - GFP - SV40"? - (Jan/02/2013 )

Dear,
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.

-lioiviei14-

lioiviei14 on Thu Jan 3 03:25:21 2013 said:


Dear,
For expression of GFP, one used 5'UTR - GFP - SV40 this sequence. What is the role of SV40 sequence in "5'UTR - GFP - SV40"?
Thank you in advance.


It helps to add the polyA tail to the upstream sequence and acts as a transcriptional terminator sequence. Here is a recent paper describing its effects:

http://www.ncbi.nlm.nih.gov/pubmed/22306880

-JYaron-