How to graph or plot MTT assay data - (Jan/01/2013 )
DRT on Sun Mar 10 18:57:10 2013 said:
Peniel on Fri Mar 8 11:01:42 2013 said:
Hi Ms W,
Just a quick question. When you do your MTS assay do you use 96wells plates. If yes, do you change the media everyday. I find out that in 72hrs the media only wells are almost empty. I would love any tips as this is my first time with this type of experiment. Thanks
A problem with the humidity levels in the incubator?
Are all your media controls in a row along one side? Be wary of always running your experiments in the same direction across a plate. In theory it shouldn't make a difference and all wells are identical but there is always the potential for biasing the results.
I don't know if the humidity level is a problem. Yeah I run my experiment in a column (B2-G2) for a drug concentration and B3-G3..... The media is usually just to fill up the empty wells.
Do you think having the wells low on media could affect my result? I assumed the plate reader only take A1(ddH20) into consideration whilst generating absorbances.
-Peniel-