Can i use spectrophotometer to replace microplate reader - (Dec/11/2012 )

I wanna run a monooxygenase titration assay and measure the content of haem. However, i dont have a microplate reader with 650 nm wavelength (the highest i have is 595nm). Is it alright if i use a regular spectrophotometer? Will there be any concern on the data obtained if i do so?

-hianghao-

I guess you can use spectrophotometer for it.

See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7

But how many samples you will be doing and time gap you need to think of.

-Inbox-

So long as you aren't measuring fluorescence of the haem, then you should be fine.

-bob1-

prabhubct on Tue Dec 11 12:55:21 2012 said:

I guess you can use spectrophotometer for it.

See http://www.protocol-...de3-ripl-cells/
Ascacioc post #7

But how many samples you will be doing and time gap you need to think of.

bob1 on Tue Dec 11 19:32:21 2012 said:

So long as you aren't measuring fluorescence of the haem, then you should be fine.

Ya, the more important part is the time frame. Thank you for the advises!

-hianghao-