Real time PCR melting curve - (Dec/03/2012 )
Actually, i face a problem with real time PCR of miRNA. I tried the primer of one type of miRNA two time and i observed a one typical bell shape curve and other multi peak curve . Could any one tell me about the causes of multi peak melting curve in real time PCR
-Mohamed 1984-
Mohamed 1984 on Tue Dec 4 01:31:40 2012 said:
Actually, i face a problem with real time PCR of miRNA. I tried the primer of one type of miRNA two time and i observed a one typical bell shape curve and other multi peak curve . Could any one tell me about the causes of multi peak melting curve in real time PCR
-casandra-
Actually, I ordered the miRNA primers directly from company. I do not formulate it.
-Mohamed 1984-